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Updated: Aug 15, 2026

Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae
Published on: February 16, 2015
Glucose deprivation mediates interaction between CTDK-I and Snf1 in Saccharomyces cerevisiae
Benoit Van Driessche1, Séverine Coddens, Vincent Van Mullem
1Unité de Recherche en Biologie Moléculaire, University of Namur, Belgium. benoit.vandriessche@fundp.ac.be
Abstract:
Ctk1 is a kinase involved in transcriptional control. We show in the two-hybrid system that Ctk1 interacts with Snf1, a kinase regulating glucose-dependent genes. Co-purification experiments confirmed the two-hybrid interaction but only when cells were grown at low glucose concentrations. Deletion of Ctk1 or its associated partners, Ctk2 and Ctk3, conferred synthetic lethality with null mutants of Snf1 or Snf1-associated proteins. Northern blot analysis suggested that Ctk1 and Snf1 act together in vivo to regulate GSY2. These findings support the view that Ctk1 interacts with Snf1 in a functional module involved in the cellular response to glucose limitation.
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