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Updated: Aug 15, 2026

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
Published on: December 1, 2020
Structural determinants of unexpected agonist activity in a retro-peptide analogue of the SDF-1alpha N-terminus
Pasquale Palladino1, Barbara Tizzano, Carlo Pedone
1Dipartimento delle Scienze Biologiche, CIRPeB, Università Federico II di Napoli, CNR, Italy. palladin@chemistry.unina.it
Abstract:
We have synthesised two retro-peptide analogues of the stromal cell derived growth factor 1 (SDF-1alpha) segment known to be critical for CXCR4 receptor binding, corresponding to the sequences HSEFFRCPCRFFESH and HSEFFRGGGRFFESH. We have assayed the ability of these peptides to activate extracellular signal-regulated kinase 1/2 phosphorylation in cells over expressing the SDF-1alpha receptor, finding that the first variant was able to serve as an agonist of CXCR4, whereas the second one was inactive. Finally, by comparing representative solution structures of the two peptides, we have found that the biological response of HSEFFRCPCRFFESH may be ascribed to a beta-beta-type turn motif centred on Phe(4)-Phe(5).
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