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Updated: Aug 15, 2026

The Multifaceted Benefits of Protein Co-expression in Escherichia coli
Published on: February 5, 2015
Expression in Escherichia coli and in vitro refolding of the human protein pLG72
Gianluca Molla1, Mariagrazia Bernasconi, Silvia Sacchi
1Department of Biotechnology and Molecular Sciences, University of Insubria, Via J. H. Dunant, 3, 21100 Varese, Italy. gianluca.molla@uninsubria.it
Abstract:
Recently, genes coding for pLG72 and d-amino acid oxidase have been related to schizophrenia, a widespread psychiatric disorder that affects about 1% of population. pLG72 is a puzzling, novel protein present only in primates and proposed to be an activator of d-amino acid oxidase. Here we report on the overexpression of wild-type and His-tagged pLG72 in Escherichia coli. Both variants form inclusion bodies and have been refolded and purified to homogeneity: the acquisition of secondary and tertiary structure was demonstrated by CD spectroscopy. A figure of approximately 70 mg of pure protein per liter of fermentation broth was achieved.
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