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Updated: Aug 15, 2026
Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages
Published on: August 12, 2019
Phenotypic, functional, and quantitative characterization of canine peripheral blood monocyte-derived macrophages
R Bueno1, M N Mello, C A S Menezes
1Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
Abstract:
The yield as well as phenotypic and functional parameters of canine peripheral blood monocyte-derived macrophages were analyzed. The cells that remained adherent to Teflon after 10 days of culture had high phagocytic activity when inoculated with Leishmania chagasi. Flow cytometric analysis demonstrated that more than 80% of cultured cells were positive for the monocyte/macrophage marker CD14.
