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Detection of Anisakis simplex-induced basophil activation by flow cytometry
Miguel Gonzalez-Muñoz1, Rosario Luque, Frans Nauwelaers
1Department of Immunology, H. Carlos III, Madrid, Spain. mgonzalez_munoz@hotmail.com
Cytometry. Part B, Clinical Cytometry
|September 27, 2005
Summary
Flow cytometry detection of Anisakis-induced basophil activation is a valuable tool for diagnosing anisakidosis. This method complements existing specific IgE tests, offering improved accuracy in identifying Anisakis allergy.
Area of Science:
- Immunology
- Parasitology
- Allergy Diagnostics
Background:
- Anisakidosis diagnosis relies on specific serum IgE.
- Basophil activation tests (BAT) using flow cytometry are emerging tools for allergy diagnosis.
Purpose of the Study:
- To assess the utility of Anisakis-induced basophil activation detection via flow cytometry for diagnosing Anisakis allergy.
Main Methods:
- Compared Anisakis allergy patients (A.s.+, n=37), non-allergic patients (A.s.-, n=51), and controls (n=12).
- Measured specific IgE to Anisakis simplex (A. simplex) via CAP-FEIA.
- Performed basophil activation tests using A. simplex crude extract and measured CD63 expression via flow cytometry.
Main Results:
- A.s.+ patients had higher age and total IgE.
- Specific IgE to A. simplex strongly correlated with basophil activation percentages (r=0.80-0.84).
- Basophil activation test showed high sensitivity (95-100%) and specificity (96-100%) for diagnosing Anisakis allergy.
Conclusions:
- Flow cytometry detection of Anisakis-induced basophil activation is a useful diagnostic technique for anisakidosis.
- This method supplements traditional specific IgE testing for Anisakis allergy.