DNA makes GFP shine

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Fluorescence Labeling to Visualize Low-Expressed Proteins in Zebrafish
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Fluorescence Labeling to Visualize Low-Expressed Proteins in Zebrafish

Published on: January 24, 2025

Irene Kaganman

    Nature Methods
    |September 28, 2005
    PubMed

    Abstract:

    An oligomerization-dependent system comprising two fragments of green fluorescent protein (GFP) fused to engineered zinc fingers could be a means to detect virtually any double-stranded DNA sequence.

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    Reporter Genes02:11

    Reporter Genes

    Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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