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Menkes protein localization in rat parotid acinar cells
Fabio D'Amico1, Evangelia Skarmoutsou, Santo Sanfilippo
1CMEM-Medical Electron Microscopy Unit, Univeristy of Catania, Via Androne 87/A, I-95124 Catania, Italy.
Abstract:
The aim was to study the subcellular localization of the Menkes protein (MNK; ATP7A) in the rat parotid acinar cell. MNK protein is a copper transporting P-type ATPase whose absence or dysfunction causes a fatal neurodegenerative disorder, MNK disease. Rat parotid glands were fixed and low-temperature embedded in Lowicryl K4M resin, and ultrathin sections were prepared for immunocytochemical analysis. Immunolocalization of MNK was demonstrated mainly over the trans Golgi network (TGN) area. Immature and mature secretory granules were also labelled, indicating that MNK protein could be involved here in copper secretion from acinar cells into saliva, consistent with a proposed cariostatic role for copper.
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