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Updated: Aug 11, 2026
Injection of Syngeneic Murine Melanoma Cells to Determine Their Metastatic Potential in the Lungs
Published on: May 24, 2016
[Experimental model of primary cultured metastatic melanoma by fine needle aspiration]
Andrea Fernandes de Oliveira1, Alfredo Gragnani, Renato Santos Oliveira Filho
1Laboratório de Cultura de Células, Universidade Federal de São Paulo, Escola Paulista de Medicina (UNIFESP/EPM), São Paulo. drandreafernandes@terra.com.br
Purpose:
Establish an experimental model of metastatic melanoma primary culture cells from fine-needle aspiration biopsies (FNAB).
Methods:
Two metastatic melanoma primary culture cells from (FNAB) have been developed from patients who had been submitted to excision of metastatic lesions and they were identified by immunohistochemical analyses using S-100 and MB-45 melanoma markers.
Results:
The culture diagnostic was confirmed by immunohistochemical. The technique of FNAB offers the advantage of providing a sequential analysis of the same tumor nodules throughout treatment, and is a minimally invasive procedure with almost no associated morbidity.
Conclusion:
The establishment of metastatic melanoma primary culture from FNAB samples showed a viable technique.
