Heat shock protein 27 and alpha B-crystallin can form a complex, which dissociates by heat shock

We have previously demonstrated that in non-oncogenic adenovirus-transformed baby rat kidney cells a complex of hsp27 and a 22-kDa protein is present, which is lacking in oncogenic cells (Zantema, A., de Jong, E., Lardenoije, R., and van der Eb, A. J. (1989) J. Virol. 63, 3368-3375). Here we show that the 22-kDa protein is identical to alpha B-crystallin. The complex of hsp27 and alpha B-crystallin is also found in some other (non-transformed) cells. However, in most cells tested only hsp27 and no alpha B-crystallin is synthesized. Gel filtration studies show that both proteins are present almost exclusively in a 700-kDa complex. Heat treatment makes the complex fall apart, which is accompanied by a change in the conformation of alpha B-crystallin. Upon recovery, complexes are formed again from both pre-existing and newly synthesized proteins.