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Monitoring Stub1-Mediated Pexophagy
Published on: May 12, 2023
[Identification of protein peroxiredoxin 2 related to crystalline NiS-induced neoplastic transformation]
Wei-dong Ji1, Jia-kun Chen, Jia-chun Lü
1Institute for Chemical Carcinogenesis, Guangzhou Medical College, Guangzhou 510182, China.
Objective:
To provide evidence for illustrating the molecular mechanism of nickel carcinogenesis, and to identify the differential expression of protein in crystalline NiS-induced neoplastic transformation of human bronchial epithelial cell by proteomics technology.
Methods:
Two dimensional electrophoresis (2-DE) and the ImageMaster 3.10 software were used to analyze the differential expression of protein, matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) combined with database search was applied to identify protein peroxiredoxin 2 (PDX2) related to malignant transformation.
Results:
The good 2-DE pattern including resolution and reproducibility was obtained. Nearly 700 expressed proteins per 2-D gel were isolated with molecular weights (MW) ranging from 14,400 to 94,000 KD and pI 3 - 10. A protein PDX2 with MW 21,890 KD, pI 5.66, which was highly expressed in malignantly transformed cell, was identified using MALDI-TOF-MS.
Conclusion:
PDX2 was involved in malignant transformation of human bronchial epithelial cell induced by crystalline nickel sulfide.
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