Related Experiment Video
Updated: Aug 15, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Published on: August 20, 2021
Reconstruction of ancient genome and gene order from complete microbial genome sequences
Yuhong Wang1, Wei Li, Tongtong Zhang
1Department of Molecular Biology, Jilin University, Changchun 130023, PR China. yuhong_wang@yahoo.com
Abstract:
Microbial genome sequences provide us with the fossil records for inferring their origination and evolution. Assuming that current microbial genomes are the evolutionary results of ancient genomes or fragments and the neighboring genes in ancient genomes are more likely neighbors in current genomes, in this paper we proposed a paleontological algorithm and assembled the orthologous gene groups from 66 complete and current microbial genome sequences into a pseudo-ancient genome, which consists of continuous fragments of various sizes. We performed bootstrap resampling and correlation analyses and the results showed that the assembled ancient genome and fragments are statistically significant and the genes of the same fragment are inherently related and likely derived from common ancestors. This method provides a new computational tool for studying microbial genome structure and evolution.
Related Concept Videos
Genome Annotation and Assembly
Evolution of Microbial Genome
Microbial Phylogeny
Evolutionary Relationships through Genome Comparisons
Modern Molecular Taxonomy
Genomics

