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Related Experiment Videos

Bves modulates epithelial integrity through an interaction at the tight junction.

Megan E Osler1, Min S Chang, David M Bader

  • 1Stahlman Cardiovascular Laboratories, Program for Developmental Biology, Division of Cardiovascular Medicine, Vanderbilt University, 222 Pierce Avenue, Nashville, TN 37232-6300, USA.

Journal of Cell Science
|September 29, 2005
PubMed
Summary

Blood vessel/epicardial substance (Bves) is a transmembrane protein that maintains epithelial integrity by interacting with tight junctions. This study reveals Bves

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Area of Science:

  • Cell Biology
  • Epithelial Biology
  • Protein Function

Background:

  • Blood vessel/epicardial substance (Bves) is a transmembrane protein found in the epicardium.
  • Its role in epithelial cell adhesion and integrity was previously unknown.
  • Bves localizes to cell-cell contacts and exhibits properties of a cell adhesion molecule.

Purpose of the Study:

  • To investigate the function of Bves in epithelial cell integrity.
  • To determine if Bves plays a role at cell junctions, specifically tight junctions (TJs).

Main Methods:

  • Localization studies of Bves with TJ markers (ZO-1, occludin) in cell lines and in vivo.
  • Biochemical assays (GST pull-down) to assess protein interactions.
  • Functional assays involving Bves knockdown to evaluate effects on epithelial integrity (transepithelial resistance, protein localization).

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Main Results:

  • Bves localizes with ZO-1 and occludin at TJs in polarized epithelial cells.
  • Bves interacts with ZO-1.
  • Bves knockdown disrupts TJ integrity, leading to decreased transepithelial resistance and altered junction protein localization.

Conclusions:

  • Bves plays a crucial role in maintaining epithelial integrity.
  • Bves functions at the tight junction, interacting with ZO-1.
  • This study identifies a novel function for Bves in epithelia.