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Related Experiment Videos

Complete nucleotide sequence of polyomavirus SA12.

Paul Cantalupo1, Adrienne Doering, Christopher S Sullivan

  • 1Department of Biological Sciences, University of Pittsburgh, PA 15260, USA.

Journal of Virology
|September 29, 2005
PubMed
Summary

Researchers sequenced simian agent 12 (SA12), a polyomavirus found in baboons. This virus is closely related to human BK virus (BKV), suggesting potential cross-species transmission and aiding in the development of diagnostic tools.

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Area of Science:

  • Virology
  • Genomics
  • Molecular Biology

Background:

  • Polyomaviridae are small viruses with circular DNA genomes infecting diverse hosts.
  • Some polyomaviruses are oncogenic in animal models or natural hosts.
  • Simian agent 12 (SA12) is a polyomavirus with a suspected natural host in chacma baboons (Papio ursinus).

Purpose of the Study:

  • To determine the complete nucleotide sequence of the SA12 genome.
  • To analyze the genetic organization and identify viral gene products.
  • To investigate the relationship between SA12 and other known polyomaviruses.

Main Methods:

  • Whole-genome sequencing of SA12.
  • Bioinformatic analysis of the genome to identify coding sequences.
  • Sequence comparison with related polyomaviruses.

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  • Development of a real-time PCR assay for differentiation.

    • Main Results:

    • The complete 5,230-bp SA12 genome sequence was determined, exhibiting typical polyomavirus genetic organization.
    • Sequences for large T antigen, small t antigen, agnoprotein, and capsid proteins (VP1, VP2, VP3) were identified.
    • SA12 expresses microRNAs from its late DNA strand, similar to SV40.
    • Sequence analysis revealed SA12 is most closely related to the human BK virus (BKV).
    • A real-time PCR assay was developed to differentiate SA12 from BKV, JC virus, and SV40.

    Conclusions:

    • The genetic makeup of SA12 is characteristic of the Polyomaviridae family.
    • The close genetic relationship between SA12 and BKV suggests potential zoonotic transmission between baboons and humans.
    • The developed PCR assay is crucial for distinguishing these closely related viruses for epidemiological and diagnostic purposes.