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Marsh stars in Liverpool.
1Department of Pathology, Royal Liverpool University Hospitals NHS Trust, Duncan Building, Daulby Street, Liverpool L69 3GA, UK.
Journal of Clinical Pathology
|September 29, 2005
Summary
Algal contamination, identified as Staurastrum, was found on histopathology slides. The likely source was the laboratory
Area of Science:
- Histopathology
- Microbiology
- Environmental Contamination
Background:
- Algal contamination on histopathological sections can mimic or obscure pathological findings.
- Periodic acid Schiff (PAS) and Grocott stains are commonly used but susceptible to certain contaminants.
Purpose of the Study:
- To investigate the source and extent of algal contamination on stained histopathological and cytological preparations.
- To identify the contaminating organism and determine its origin within the laboratory environment.
Main Methods:
- Recognition of contaminating algae on PAS and Grocott stained slides.
- Detailed audit of laboratory procedures and water sources.
- Microscopic identification of the contaminating organism.
Main Results:
- The contaminant was identified as Staurastrum, a genus of Chlorophyta (green algae).
- Algae were observed on sections stained with diastase-PAS, PAS, and Grocott methods between July 2003 and May 2004.
- Contamination of water baths or staining solutions was ruled out; a general laboratory water supply contamination is the most probable source.
Conclusions:
- Investigating the nature and source of contaminants in histological and cytological samples is crucial.
- While this specific algal contamination caused minimal diagnostic confusion, algae can occasionally be significant pathogens.
- Implementing measures to ensure water quality is essential for accurate histopathological diagnosis.