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Updated: Aug 15, 2026

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
Published on: May 19, 2023
Rosiglitazone treatment increases subcutaneous adipose tissue glucose uptake in parallel with perfusion in patients
Antti P M Viljanen1, Kirsi A Virtanen, Mikko J Järvisalo
1PET Centre, Turku University Central Hospital, Turku, Finland.
Context:
We have shown that rosiglitazone increases whole-body and adipose tissue insulin sensitivity in humans.
Objective:
The aim of this study was to further examine whether possible changes in adipose perfusion could explain increased adipose tissue glucose uptake (GU).
Patients:
Thirty-seven patients with newly diagnosed type 2 diabetes were included.
Intervention:
Patients were randomized into treatment with rosiglitazone, metformin, or placebo for 26 wk in a double-blinded trial.
Design:
Femoral adipose flow and GU were measured with [15O]H2O, [18F]fluorodeoxyglucose and positron emission tomography during euglycemic hyperinsulinemia. Adipose masses were measured using magnetic resonance imaging.
Results:
Metformin and rosiglitazone treatment improved glycemic control, but only rosiglitazone increased whole-body insulin sensitivity. Rosiglitazone treatment increased flow by 72% (P < 0.01) and GU by 23% (P < 0.05) and thereby decreased adipose tissue glucose extraction by 18% (P < 0.05); no changes were observed in the metformin or placebo-treated groups. When the adipose masses were taken into account, rosiglitazone treatment increased flow by 73% (P < 0.01) and GU by 24% (P < 0.05). During hyperinsulinemia, flow correlated with GU (r = 0.63; P < 0.01).
Conclusions:
In conclusion, s.c. GU is associated with flow in patients with type 2 diabetes. Rosiglitazone treatment enhances GU and flow but decreases glucose extraction, suggesting that perfusion may contribute to adipose tissue insulin sensitization by rosiglitazone.
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