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Exogenous 5'-nucleotidase improves glomerular autoregulation in Thy-1 nephritic rats
Tsuneo Takenaka1, Hirokazu Okada, Yoshihiko Kanno
1Department of Nephrology, Saitama Medical College, Iruma Saitama 350-0495, Japan.
American Journal of Physiology. Renal Physiology
|September 29, 2005
Summary
Renal autoregulation is impaired in Thy-1 nephritis, affecting renal plasma flow and glomerular filtration rate. Prostanoids and mesangial cells, possibly via 5'-nucleotidase, play roles in maintaining renal circulation and autoregulation.
Area of Science:
- Nephrology
- Renal Physiology
Background:
- Thy-1 nephritis is a model of mesangiolysis.
- Renal autoregulation is crucial for maintaining kidney function.
- Understanding hemodynamic changes in nephritis is vital for treatment.
Purpose of the Study:
- To investigate renal hemodynamic responses in Thy-1 nephritic rats.
- To determine the role of tubuloglomerular feedback, 5 -nucleotidase, and prostanoids in renal autoregulation during nephritis.
Main Methods:
- Induction of Thy-1 nephritis in rats using a monoclonal antibody.
- Assessment of renal plasma flow (RPF) and glomerular filtration rate (GFR) under varying blood pressures.
- Administration of furosemide, albumin, 5 -nucleotidase, and indomethacin to evaluate their effects on renal hemodynamics and autoregulation.
Main Results:
- Renal autoregulation of RPF and GFR was impaired in nephritic rats.
- Inhibition of tubuloglomerular feedback affected autoregulation in control but not nephritic rats.
- 5 -nucleotidase infusion ameliorated GFR autoregulatory decrements in nephritic rats.
- Prostaglandin synthesis inhibition (indomethacin) reduced RPF and GFR but did not restore autoregulation.
Conclusions:
- Renal autoregulation is significantly impaired in Thy-1 nephritis.
- Prostanoids are important for maintaining renal circulation in nephritic rats.
- Mesangial cells and/or 5 -nucleotidase are implicated in mediating renal autoregulation during nephritis.