Related Experiment Video
Updated: Aug 15, 2026

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
Microbial transformations 59: first kilogram scale asymmetric microbial Baeyer-Villiger oxidation with optimized
Iris Hilker1, Roland Wohlgemuth, Véronique Alphand
1Groupe Biocatalyse et Chimie Fine, FRE CNRS 2712, Université de la Méditerranée, Faculté des Sciences de Luminy, Case 901, 163 avenue de Luminy, 13288 Marseille Cedex 9, France.
Abstract:
This study is demonstrating the scale up of asymmetric microbial Baeyer-Villiger oxidation of racemic bicyclo[3.2.0]hept-2-en-6-one (1) to the kilogram scale using a 50 L bioreactor. The process has been optimized with respect to bottlenecks identified in downscaled experiments. A high productivity was obtained combining a resin-based in situ substrate feeding and product removal methodology (in situ SFPR), a glycerol feed control, and an improved oxygenation device (using a sintered-metal sparger). As expected both regioisomeric lactones [(-)-(1S,5R)-2 and (-)-(1R,5S)-3] were obtained in nearly enantiopure form (ee > 98%) and good yield. This represents the first example of such an asymmetric Baeyer-Villiger biooxidation reaction ever operated at that scale. This novel resin-based in situ SFPR technology therefore clearly opens the way to further (industrial) upscaling of this highly valuable (asymmetric) reaction.
Related Concept Videos
Scale-Up Processes
Microbial Wastewater Treatment
Bioreactor Controls-III
Production of Antibiotics
Production of Organic Acids
Microbial Bioremediation of Pesticides

