Related Experiment Video
Updated: Aug 15, 2026
A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene
Published on: April 1, 2019
[The relevance of tumor necrosis factor-alpha gene-863C/A polymorphism with thyroid-associated ophthalmopathy]
1Department of Endocrinology, the Qingdao University Medical School Hospital, Qingdao 266003, China. yansl2001@sohu.com
Objective:
To investigate the relevance of tumor necrosis factor-alpha (TNF-alpha) gene -863C/A polymorphism with thyroid-associated ophthalmopathy (TAO).
Methods:
TNF-alpha gene polymorphism at position -863 was determined by PCR-RFLP in 76 normal people, 54 patients with TAO and 60 patients with autoimmune thyroid disease (AITD) who had no ophthalmopathy. All the subjects were collected from July 2002 to December 2003 in out-patient department of endocrinology in the hospital. The difference of genotype and the variation of allele frequencies were analyzed by Chi-square test.
Results:
(1) Frequencies distribution of CA + AA genotype in TAO, non-ophthalmopathy and control groups were 46.3%, 30.0%, 25.0% respectively, and allele A were 27.8%, 15.0%, 12.5% for those three groups respectively. (2) Frequencies of allele A in TAO group were significantly higher than those in non-ophthalmopathy and control groups (P = 0.018 and 0.002 respectively). (3) When the three groups were stratified according to sex, frequencies of -863 CA + AA genotype and allele A in male TAO patients were significantly higher than those in control group (OR = 4.31, P = 0.019; OR = 4.81, P = 0.003) and non-ophthalmopathy group (OR = 4.87, P = 0.027; OR = 5.38, P = 0.008). No significant difference was found in female patients (P > 0.05). (4) Frequencies of CA + AA genotype and allele A in TAO patients with 5 + 6 grade were significantly higher than those in non-ophthalmopathy group (CA + AA genotype: OR = 20.68, P = 0.021; allele A: OR = 39.67, P < 0.001).
Conclusion:
Allele A of TNF-alpha gene at position -863 may be associated with TAO especially in male patients.
Related Concept Videos
01:11Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase
01:31Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
01:31Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
01:20Synthesis and Regulation of Thyroid Hormones
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The iodine is then...
01:11Genetic Lingo