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Reaction of macrophage disappearance as a method for the evaluation of cellular immunity in experimental plague
G I Vasilieva1, A K Kisileva, Y P Doroshenko
1Anti-Plague Research Institute, Rostov-on-Don, USSR.
Abstract:
The reaction of macrophage disappearance (RMD) was found to develop one day after the immunization of experimental animals with the antigen FIA of Y. pestis and to persist for 21 days, correlating with a pronounced protective effect of the above antigen over this period. There were differences between white mice and guinea pigs in the intensity of the RMD, particularly when immunization was carried out using an FIA preparation without prolonged action. These differences are thought to reflect varying degrees of immunity elicited by FIA immunization. It is recommended that the RMD be used to quantify cellular immunity to Y. pestis.
Insights
The reaction of macrophage disappearance (RMD) effectively quantifies cellular immunity to Y. pestis post-immunization. This immune response correlates with protection and varies between animal models.
Area of Science:
- Immunology
- Microbiology
- Veterinary Science
Background:
- Yersinia pestis (Y. pestis) infection poses a significant public health threat.
- Effective vaccines and diagnostic tools for Y. pestis are crucial for disease control.
- Cellular immunity plays a vital role in protection against Y. pestis.
Purpose of the Study:
- To investigate the kinetics and utility of the reaction of macrophage disappearance (RMD) as a measure of cellular immunity.
- To assess the correlation between RMD and protective immunity induced by Y. pestis Fraction 1 antigen (FIA).
- To evaluate interspecies differences in RMD intensity between mice and guinea pigs.
Main Methods:
- Experimental animals (mice and guinea pigs) were immunized with Y. pestis FIA.
- The development and duration of RMD were monitored post-immunization.
- Protective effects were assessed over a 21-day period.
- RMD intensity was compared between different animal models and immunization preparations.
Main Results:
- RMD was observed one day after immunization and persisted for 21 days.
- The duration of RMD correlated with a pronounced protective effect against Y. pestis.
- Significant differences in RMD intensity were noted between mice and guinea pigs, suggesting varying immune responses.
Conclusions:
- RMD is a reliable indicator of cellular immunity elicited by Y. pestis FIA immunization.
- The intensity of RMD reflects the degree of immunity conferred by FIA.
- RMD serves as a valuable tool for quantifying cellular immunity to Y. pestis.