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Reusable impedimetric aptasensor.

Abd-Elgawad Radi1, Josep Lluís Acero Sánchez, Eva Baldrich

  • 1Department of Chemistry, Faculty of Science, Mansoura University, 34517 Dumyat, Egypt. abd.radi@urv.net

Analytical Chemistry
|October 1, 2005
PubMed
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This study introduces a novel impedimetric aptasensor for detecting thrombin. The sensor, utilizing a mixed self-assembled monolayer on a gold electrode, demonstrates efficient thrombin detection and regeneration capabilities.

Area of Science:

  • Biosensor technology
  • Electrochemical sensing
  • Biomolecular interaction analysis

Background:

  • Thrombin is a key biomarker in various physiological and pathological processes.
  • Development of sensitive and selective biosensors is crucial for accurate thrombin quantification.
  • Impedimetric aptasensors offer label-free detection methods with potential for high sensitivity.

Purpose of the Study:

  • To develop and characterize a novel impedimetric aptasensor for thrombin detection.
  • To investigate the sensor's performance, including sensitivity, selectivity, and regeneration.
  • To explore the use of a mixed self-assembled monolayer for enhanced sensor performance.

Main Methods:

  • Fabrication of a gold electrode modified with a mixed self-assembled monolayer of thrombin binding aptamer and 2-mercaptoethanol.

Related Experiment Videos

  • Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) for probing interfacial changes.
  • Regeneration of the aptasensor using a high salt concentration solution (2.0 M NaCl).

    • Main Results:

    • The aptasensor exhibited an increase in interfacial electron-transfer resistance upon thrombin binding.
    • A linear response of charge-transfer resistance to thrombin concentration was observed in the range of 5.0–35.0 nM.
    • The sensor demonstrated a low detectable concentration of thrombin down to 2.0 nM.
    • Successful regeneration of the aptasensor for repeated thrombin detection was achieved.

    Conclusions:

    • The developed impedimetric aptasensor is a sensitive and reusable platform for thrombin detection.
    • The mixed self-assembled monolayer strategy enhances sensor performance.
    • This aptasensor shows promise for clinical diagnostics and biomedical research applications.