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Updated: Aug 15, 2026

A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer
Published on: July 6, 2017
[Radiosensitivity on colorectal neoplasms by recombinant adenoviral-mediated wild-type p53 gene]
Bin-bin Cui1, Ming Liu, Peng Zhao
1Department of Abdominal Surgery, The Affiliated Tumor Hospital of Harbin Medical University, Harbin 150040, China. 0451cbb@163.com
Objective:
To study the radiosensitization on the cells of colorectal cancer transfected with recombinant adenovirus vector-mediated wild-type p53.
Methods:
SW480 cells transfected by wild-type p53 were treated with 4 Gy and 6 Gy radiation. The expression of recombinant adenovirus vector-mediated wild-type p53 gene was detected by Western blotting. The inhibition rate of SW480 cells was examined by MTT, apoptotic rate by TdT-mediated dUTP nick end labeling (TUNEL) and proliferating cell nuclear antigen (PCNA) by immunohistochemical method.
Results:
SW480 cells transfected by wild-type p53 were inhibited significantly by 4 Gy and 6 Gy radiation. The level of apoptosis increased and the expression of PCNA decreased.
Conclusion:
Cells of colorectal carcinoma transfected with wild-type p53 increases their radiation sensitivity.
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