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Related Experiment Videos

Insulin-like growth factor II expression in the developing human brain.

P A McKelvie1, K M Rosen, H C Kinney

  • 1Department of Neurology, Children's Hospital, Boston, Massachusetts 02115.

Journal of Neuropathology and Experimental Neurology
|July 1, 1992
PubMed
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Insulin-like growth factor II (IGF-II) mRNA is widely expressed in the developing human brain, particularly in the choroid plexus and leptomeninges. Its expression declines in brain parenchyma after birth, supporting a role in early neural development.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Molecular Biology

Background:

  • Insulin-like growth factor II (IGF-II) is a polypeptide hormone with known metabolic and neurotrophic activities.
  • IGF-II has been implicated in human brain development.
  • Understanding IGF-II expression patterns is crucial for elucidating its role in neurodevelopment.

Purpose of the Study:

  • To examine the spatial and temporal patterns of IGF-II mRNA expression in the developing human brain.
  • To investigate the developmental trajectory of IGF-II mRNA in various brain regions from midgestation to early childhood.

Main Methods:

  • Northern blot analysis was employed to detect IGF-II mRNA expression.
  • The study analyzed 18 human brain samples across a developmental range from 20 gestational weeks to 2.5 years.

Related Experiment Videos

  • Specific brain regions including leptomeninges, choroid plexus, and parenchyma were examined.

    • Main Results:

    • IGF-II mRNA expression was widespread in the brain from midgestation through the perinatal period.
    • Expression levels and developmental patterns varied significantly across different brain regions.
    • Leptomeninges and choroid plexus showed the highest sustained IGF-II mRNA levels.
    • Parenchymal IGF-II mRNA expression decreased significantly after two postnatal months, persisting mainly in the choroid plexus.
    • Both 6.0 and 4.8 kb IGF-II transcripts were detected, with developmental changes in their relative abundance in certain regions.

    Conclusions:

    • The widespread expression of IGF-II mRNA in the developing brain parenchyma during late gestation supports its role in stimulating neural cell proliferation and differentiation.
    • Distinct regional and temporal expression patterns suggest a complex, stage-specific function of IGF-II in human brain development.
    • The persistence of IGF-II expression in the choroid plexus postnatally may indicate ongoing roles beyond early neurogenesis.