Related Experiment Video
Updated: Aug 15, 2026

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization
Published on: June 28, 2012
A gene-based radiation hybrid map of chicken microchromosome 14: comparison to human and alignment to the assembled
Mireille Morisson1, Sophie Leroux, Carine Jiguet-Jiglaire
1Laboratoire de génétique cellulaire, Institut national de la recherche agronomique, Castanet-Tolosan, France. mmorisso@toulouse.inra.fr
Abstract:
We present a gene-based RH map of the chicken microchromosome GGA14, known to have synteny conservations with human chromosomal regions HSA16p13.3 and HSA17p11.2. Microsatellite markers from the genetic map were used to check the validity of the RH map and additional markers were developed from chicken EST data to yield comparative mapping data. A high rate of intra-chromosomal rearrangements was detected by comparison to the assembled human sequence. Finally, the alignment of the RH map to the assembled chicken sequence showed a small number of discordances, most of which involved the same region of the chromosome spanning between 40.5 and 75.9 cR(6000) on the RH map.
More Related Videos
10:14Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice
Published on: January 11, 2017
12:47Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples
Published on: August 29, 2017
Related Concept Videos
Karyotyping
Synteny and Evolution
Around 80 million years ago, the human and mice lineages diverged from the common ancestor. During the course of evolution, the ancestral chromosome underwent...
DNA Microarrays
Heterochromatin
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...