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Updated: Aug 15, 2026

MISSION esiRNA for RNAi Screening in Mammalian Cells
Published on: May 12, 2010
Induction of stable RNA interference in mammalian cells
1Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA. culle002@mc.duke.edu
Abstract:
Over the last years, RNA interference (RNAi) has become a widely used technique that permits the knock-down, and hence functional analysis, of individual genes in vertebrate cells. However, the high failure rate of the RNA molecules used in RNAi experiments continues to be a problem. In this paper, I describe a set of design criteria, experimental steps and expression vectors that can facilitate the effective knock-down of almost any vertebrate gene product in cultured cells or in experimental animals.Gene Therapy (2006) 13, 503-508. doi:10.1038/sj.gt.3302656; published online 29 September 2005.
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