Comparison of growth rates of fresh and frozen-thawed embryos according to chromosomal status
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Updated: Aug 15, 2026
Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis
Published on: September 7, 2021
Rohini Edirisinghe1, Rodney Jemmott, John Allan
1The Wesley IVF Service, The Wesley Hospital, Auchenflower, Brisbane, Queensland, Australia. Rohini.Edirisinghe@wesley.com.au
Purpose:
To report a comparison of growth rates between fresh and frozen-thawed embryos on day 3 and 4 according to chromosomal status during PGD.
Methods:
Embryos were biopsied on day 3, fluorescent in situ hybridization performed for aneuploidy detection and growth rates monitored.
Results:
Based on 241 egg pick up-PGD and 133 frozen embryo transfer-PGD cycles. The development rate of embryos despite their chromosomal status was similar on day 3, however, overall, fresh embryos showed a significantly improved development (> or =6 cell stage; 85.7%) compared to frozen embryos (77.0%; p < 0.003). On day 4 fresh embryos showed accelerated development and further developmental improvements were associated with chromosomal normality (56.1% normal vs. 42.2% aneuploid; p < 0.004).
Conclusions:
Cryopreservation slows embryo development regardless of the chromosomal status. However, chromosomal status did not influence the chance of embryo implantation which was the same for fresh and thawed embryos.
