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Updated: Jul 15, 2026

Mass Spectrometric Analysis of Glycosphingolipid Antigens
Published on: April 16, 2013
Nanoliquid chromatography-mass spectrometry of oligosaccharides employing graphitized carbon chromatography on
Milady Niñonuevo1, Hyunjoo An, Hongfeng Yin
1Department of Chemistry, University of California, Davis, CA 95616, USA.
Abstract:
The nanoLC separations of oligosaccharides using microchip-based columns are described. Mixtures of alditols from mucins and human milk are separated on graphitized carbon. The nanoLC-MS device showed high mass accuracy for the oligosaccharides ranging between 1 and 6 ppm on routine analyses. The high mass accuracy readily allowed identification of oligosaccharide peaks and the determination of their compositions. High retention time reproducibility was exhibited by the microchip LC. Little variation was observed for standard sample either alone or in a complex heterogeneous mixture. The nanoLC-MS exhibits excellent capabilities in profiling mixtures of oligosaccharides.
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