Related Experiment Video
Updated: Aug 15, 2026

Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization
Published on: April 5, 2019
Hen egg white fractionation by ion-exchange chromatography
C Guérin-Dubiard1, M Pasco, A Hietanen
1UMR 1253 Agrocampus Rennes-INRA, Sciences et technologie du lait et de l'oeuf, 65 rue de Saint-Brieuc, CS 84215, 35042 Rennes, France. Catherine.guerin@agrocampus-rennes.fr
Abstract:
Major hen egg white proteins have been widely studied for their functional properties but these studies still are unable to explain, alone, all of the biological properties of hen egg white. Hence, it is still interesting to produce pure and non-altered proteins to improve our knowledge on the biological properties of hen egg white. Presently, identification and characterization of both bioactive peptides and minor proteins from hen egg white is essential work for progressing in the understanding of hen egg white biological properties. With this objective in mind, a new process for a complete "mucin free" hen egg white fractionation based on ion exchange chromatography is proposed. "Mucin free" egg white is fractionated into six different fractions. Four of them are high-recovery yield purified fractions of lysozyme, ovotransferrin, ovalbumin and flavoprotein. The two other fractions are enriched in recently detected minor proteins in hen egg white.
More Related Videos
06:12Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs
Published on: January 27, 2021
09:57Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
Published on: June 14, 2020
Related Concept Videos
Ion-Exchange Chromatography
Subcellular Fractionation
Differential Centrifugation
Differential centrifugation is...
Ion Exchange
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...