Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genetic toxicity studies with genistein.

R Michael McClain1, Erich Wolz, Alberto Davidovich

  • 1McClain Associates, Randolph, NJ 07869, USA. michaelmcclain@msn.com

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
|October 4, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Safety studies conducted on high-purity trans-resveratrol in experimental animals.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2009
Same author

Emil Alvin Pfitzer.

Toxicological sciences : an official journal of the Society of Toxicology·2007
Same author

Reproductive safety studies with genistein in rats.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2007
Same author

A one-year neonatal mouse carcinogenesis study of quinacrine dihydrochloride.

International journal of toxicology·2006
Same author

Acute, subchronic and chronic safety studies with genistein in rats.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2005
Same author

Exposure to retinoic acids in non-pregnant women following high vitamin A intake with a liver meal.

International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition·2005

Genistein, a soy phytoestrogen, was evaluated for mutagenicity and clastogenicity. It was not mutagenic in bacterial assays or in vivo micronucleus tests, but showed clastogenic potential in vitro mouse lymphoma assays.

Area of Science:

  • Toxicology
  • Molecular Biology
  • Nutraceuticals

Background:

  • Genistein, a soy isoflavone, is studied for potential chemopreventive properties.
  • Concerns exist regarding potential adverse effects of genistein due to its estrogenic activity.
  • Epidemiological studies suggest reduced cancer rates in populations with high soy intake.

Purpose of the Study:

  • To assess the mutagenicity and clastogenicity of genistein.
  • To evaluate genistein's safety profile for potential therapeutic applications.
  • To investigate genistein's genotoxic potential using in vitro and in vivo models.

Main Methods:

  • In vitro bacterial reverse mutation assay (Ames Test) with and without metabolic activation (S9).
  • In vitro mouse lymphoma assay with and without metabolic activation (S9).

Related Experiment Videos

  • In vivo micronucleus tests in mice and rats following oral administration.

    • Main Results:

    • Genistein showed no mutagenic effects in the Ames Test.
    • Genistein induced resistant mutants (predominantly small colonies) in the mouse lymphoma assay, indicating clastogenicity.
    • No evidence of mutagenicity or clastogenicity was observed in the in vivo micronucleus tests in mice and rats.

    Conclusions:

    • Genistein is not mutagenic in bacterial systems or in vivo.
    • Genistein exhibits clastogenic activity in vitro, likely through topoisomerase II inhibition.
    • The in vivo data suggest genistein does not pose a significant genotoxic risk at tested doses.