Related Experiment Video
Updated: Aug 15, 2026

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
Published on: November 14, 2018
Adaptor polymerase chain reaction for single molecule amplification
Michihiko Nakano1, Jun Komatsu, Hirofumi Kurita
1Department of Ecological Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku-cho, Toyohashi, Aichi 441-8580, Japan.
Abstract:
The adaptor polymerase chain reaction (PCR) permits the amplification of DNA fragments with arbitrary sequences. In this paper, we describe the successful amplification of plasmid-derived single molecule DNAs digested by a restriction enzyme. By using adaptors made of short and long oligonucleotides, nonspecific interactions during PCR were suppressed. The method will be applicable to the detection of single molecule DNA fragments even if their sequence is unknown.
Related Concept Videos
PCR
PCR - Polymerase Chain Reaction
Real Time RT-PCR
The real-time quantification of the number of amplified products is...
RACE - Rapid Amplification of cDNA Ends
Since the...

