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Updated: Aug 15, 2026

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
Published on: September 17, 2017
Novel 13C direct detection experiments, including extension to the third dimension, to perform the complete
Wolfgang Bermel1, Ivano Bertini, Isabella C Felli
1Bruker BioSpin GmbH, Rheinstetten, Germany.
Abstract:
Carbon-13 direct detection NMR methods are feasible thanks to the improvements in probehead technology and to the development of new NMR experiments. We present here a complete set of experiments, based on C' direct detection, developed to perform protein complete assignment of backbone and side-chains (except for aromatic rings). This strategy offers alternative solutions for demanding situations (paramagnetic and/or large molecules) and can be useful in general in conjunction with conventional experiments.
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