Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Protective effect of human decay accelerating factor on mouse cells].

Y Zhao1, L Li, Y Fu

  • 1Department of Urology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.

Zhonghua Yi Xue Za Zhi
|October 5, 2005
PubMed
Summary

Human decay accelerating factor (DAF) protects mouse cells from complement-mediated injury. Mouse cells expressing DAF survived in human serum, while control cells did not.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sequences flanking the pentanucleotide T-antigen binding sites in the polyomavirus core origin help determine selectivity of DNA replication.

Journal of virology·1995
Same author

Inactivation and conformation changes of the glycated and non-glycated D-glyceraldehyde-3-phosphate dehydrogenase during guanidine-HCl denaturation.

Biochimica et biophysica acta·1995
Same author

Safe separation of sputum cells from mucoid glycoprotein.

Acta cytologica·1995
Same author

Linkage between sexual orientation and chromosome Xq28 in males but not in females.

Nature genetics·1995
Same author

Dermo-1: a novel twist-related bHLH protein expressed in the developing dermis.

Developmental biology·1995
Same author

Positive regulation of human alpha 1 (I) collagen promoter activity by transcription factor Sp1.

Gene·1995

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • The complement system is a crucial part of innate immunity.
  • Complement-mediated cell lysis can be a significant challenge in biological research and therapies.

Purpose of the Study:

  • To investigate the protective role of human decay accelerating factor (DAF) against complement-induced damage.
  • To determine if expressing human DAF in mouse cells confers resistance to human complement.

Main Methods:

  • Mouse myeloma cells were genetically modified to express human DAF cDNA.
  • Transfected cells and control cells were cultured in normal human serum.
  • Cell viability and survival were monitored over time.

Main Results:

  • Mouse cells engineered to express human DAF demonstrated significant resistance to complement-mediated injury.

Related Experiment Videos

  • DAF-expressing cells survived for over 48 hours in human serum and maintained viability across generations.
  • Control mouse cells lacking DAF expression were rapidly injured and died within 1-4 hours.

    • Conclusions:

    • Human DAF effectively protects susceptible cells from the damaging effects of human complement.
    • DAF is a potent inhibitor of complement-mediated cell lysis.
    • This finding has implications for understanding complement regulation and developing cell-protective strategies.