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Oct2 transactivation from a remote enhancer position requires a B-cell-restricted activity.

A Annweiler1, M Müller-Immerglück, T Wirth

  • 1Zentrum für Molekulare Biologie Heidelberg, Germany.

Molecular and Cellular Biology
|July 1, 1992
PubMed
Summary

The transcription factor Oct2 (octamer-binding transcription factor 2) can activate gene expression via octamer motifs, but this function is specific to B cells. Its carboxy-terminal domain is crucial for this enhancer activity.

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Area of Science:

  • Molecular Biology
  • Immunology
  • Genetics

Background:

  • Ectopic expression of the lymphocyte-specific transcription factor Oct2 activates promoters with octamer motifs.
  • Oct2 fails to stimulate multimerized octamer enhancer elements in non-B cells like HeLa cells.

Purpose of the Study:

  • To investigate the capability of various Oct2 isoforms to activate an octamer enhancer in different cell types.
  • To identify the domains of Oct2 responsible for enhancer activation, particularly in nonlymphoid versus B cells.

Main Methods:

  • Cotransfection experiments with different Oct2 isoforms.
  • Analysis of Oct2 isoform activity in B cells and nonlymphoid cells.
  • Mutational analysis of Oct2 domains.
  • Domain swapping experiments between Oct1 and Oct2.

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Main Results:

  • Several Oct2 isoforms can stimulate enhancer activity from a remote position, but this is restricted to B cells.
  • B-cell-specific cofactor involvement or modification is suggested for Oct2-mediated enhancer activation.
  • The carboxy-terminal domain of Oct2 is critical for enhancer activation.
  • Transferring the carboxy-terminal domain to Oct1 confers enhancer activity.

Conclusions:

  • Oct2-mediated enhancer activation is cell-type specific, depending on B-cell-specific factors or modifications.
  • The carboxy-terminal domain of Oct2 plays a key role in mediating enhancer activation, independent of other domains like the POU or glutamine-rich regions.