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Updated: Aug 15, 2026
Practical Considerations in Studying Metastatic Lung Colonization in Osteosarcoma Using the Pulmonary Metastasis Assay
Published on: March 12, 2018
[Biological stabilities and identification of a highly metastatic subpopulation from a human osteosarcoma cell line]
1Orthopaedic Institute of People's Liberation Army, 89th Hospital, Weifang 261021, China.
Objective:
To investigate the biological stabilities and the identification of a human highly metastatic osteosarcoma subpopulation.
Methods:
Tumor pieces of the cell were inoculated into nude mice orthotopically. Lung node metastatic foci were obtained by culturing, the procedure was repeated for 3 cycles. Changes were observed in intra-tibial tumor growth, pulmonary metastatic rate in vivo, morphological characteristics, expressions of BMP, vimentin, actin, NEC and karyotype analyses of every passage in vitro.
Results:
The intra-tibial tumor growth rates were 100%, the proliferation abilities were stable, and the pulmonary metastatic rates were higher than 80%. The expressions of BMP, vimentin, actin were positive, but the expression of NSE was negative. Its morphological characteristics the antigen-positive substances and karyotype were similar to those observed in human osteosarcoma.
Conclusion:
SOSP-M1 with very stable biological characteristics is a good model for the study of human osteosarcoma.
