Related Experiment Videos
[Microsatellite marker linked with stripe rust resistant gene Yr9 in wheat]
Dong-Xu Weng1, Shi-Chang Xu, Rui-Ming Lin
1Institute of Plant Protection, Chinese Academy of Agricultural Sciences, State Key Lab of Biology for Plant Diseases and Insect Pests , Beijing 100094, China.
Yi Chuan Xue Bao = Acta Genetica Sinica
|October 6, 2005
Summary
Researchers identified a DNA marker, Xgwm582, linked to the Yr9 gene for wheat stripe rust resistance. This finding aids in developing resistant wheat varieties through marker-assisted selection.
Area of Science:
- Plant genetics
- Molecular markers
- Wheat breeding
Context:
- Wheat stripe rust poses a significant threat to global food security.
- Identifying resistance genes is crucial for developing resistant crop varieties.
- Microsatellite markers offer a powerful tool for genetic analysis in wheat.
Purpose:
- To identify and characterize microsatellite markers linked to the Yr9 gene conferring resistance to wheat stripe rust.
- To establish the genetic linkage and distance between a specific SSR marker and the Yr9 resistance gene.
Summary:
- SSR analysis was conducted on a wheat near-isogenic line (NIL) carrying the Yr9 stripe rust resistance gene and its recurrent parent.
- The microsatellite marker Xgwm582 was identified as polymorphic and linked to the Yr9 gene on chromosome 1B.
- Genetic linkage analysis in 177 F2 plants revealed a genetic distance of 3.7 cM between Xgwm582 and Yr9.
Impact:
- Facilitates marker-assisted selection for accelerating wheat breeding programs focused on stripe rust resistance.
- Provides a valuable molecular tool for pyramiding resistance genes and developing durable disease resistance in wheat.
- Contributes to the genetic understanding of wheat-stripe rust interactions and resistance mechanisms.