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Updated: Aug 15, 2026

Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA
Published on: December 15, 2023
[Serum HBV-DNA level can predict treatment effectiveness in patients with chronic hepatitis B using
Hong Zhao1, Gui-qiang Wang, Zhong-hou Han
1The Department of Infectious Diseases, The First Teaching Hospital of Beijing University, Beijing, China.
Objective:
To evaluate the correlation between the efficacy of interferon-alpha-2a and the kinetics of viral load in serum.
Methods:
The authors conducted a trial including 58 patients with chronic hepatitis B. Patients were treated with interferon-alpha-2a three times a week for 6 months. Viral kinetics were assessed by serial quantitive measurements of HBV-DNA.
Results:
A significant decline of serum HBV-DNA was seen after interferon-alpha-2a administration for 1 month, the decreases were (2.50 +/- 0.44) log10, (1.62 +/- 1.12) log10 and (1.05 +/- 1.35) log10 for complete responders, partial responders and no-responders, respectively. After 1 month of treatment, HBV-DNA level was (3.99 +/- 0.91) log10 for complete responders versus (5.63 +/- 1.31) log10 for partial responders, and (6.69 +/- 1.42) log10 for no-responders (P < 0.05). Multivariate analysis suggested that undetectable serum HBV-DNA after 1 month of interferon-alpha-2a treatment was associated with better efficacy; higher baseline ALT or/and no family history were also correlated with better treatment outcomes.
Conclusion:
Kinetics of HBV-DNA level under interferon-alpha-2a treatment are highly predictive of therapeutic response.
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