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Published on: September 25, 2019
[Expression of HBV preS2/S gene in mammalian cells transferred with adenoviral vector]
Chun-liang Lei1, Cheng-hui Huang, Zhan Yang
1The Eighth Hospital of Guangzhou, Guangzhou 510060, China.
Objective:
To study HBV preS2/S gene expression effects in mammalian cells transferred with recombinant adenoviral vector.
Methods:
The replication-deficient recombinant adenoviral vector (Ad-HBs) carrying HBV preS2/S gene were constructed by homologous recombination in bacteria. The 293 cells, Vero cells, HepG2 cells and mesenchymal stem cells (MSCs) were infected with adenoviruses. The expressions of enhanced green fluorescent protein (EGFP) were observed with fluorescence microscope and the expressions of HBsAg were detected by RT-PCR and ELISA in vitro.
Results:
More than 90% of 293 cells, Vero cells, HepG2 cells or MSCs expressed EGFP after transfection at the MOI of 20 and the titers of HBsAg were more than 3.229 (A value) in culture supernatant.
Conclusion:
The HBV preS2/S gene was not only expressed efficiently in immortalized cells, but also expressed efficiently in stem cells with the recombinant adenoviruses vector.
