Related Experiment Video
Updated: Aug 15, 2026

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids
Published on: March 17, 2016
[Establishment and application of fluorescent quantitative PCR method for detecting human herpes virus type 6]
Xue-jun Dong1, Chang-gen Shi, Shi-wen Qu
1Central Laboratory, Shaoxing People's Hospital, Shaoxing, China.
Objective:
To establish fluorescent quantitative PCR method for detecting human herpes virus type 6 (HHV6).
Methods:
According to the specific sequence of human herpes virus type 6 genes, the primers and the fluorescent probe (TaqMan) were designed and synthesized. The fragment generated from HHV 6 gene as template was cloned into the pMD18-T vector which was constructed from the pUC 18. And the positive recombinant plasmid was 1:10 diluted and used as standard quantitative template to make the standard curve for sample detection.
Results:
The standard curve indicated the linear relationship between Ct (cycle threshold) and template concentration. The clinical samples from 135 cases were detected by this system, 16 cases among 135 were positive.
Conclusion:
The fluorescent quantitative PCR method for the detection of human herpes virus type 6 is simple and accurate, and this method may be helpful to clinical diagnosis.
Related Concept Videos
Herpes
Genital Herpes

