Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Video frame interpolation for coronary angiography using latent flow matching.

NPJ digital medicine·2026

Same author

Pharmacokinetics and Renal Safety Evaluation of Colistin Methanesulfonate Sodium in Korean Healthy Male Volunteers: An Open-Label, Multiple-Dose Study.

Drug design, development and therapy·2026

Same author

PEGDA hydrogel-based biosensor for continuous salivary cortisol monitoring without pre-treatment.

Talanta·2026

Same author

Bayesian Pairwise Compositional Lotka-Volterra Modeling Infers Potential Rhizosphere Microbial Suppressors of Ralstonia pseudosolanacearum.

The plant pathology journal·2026

Same author

Pulmonary Vascular Proliferative Lesions in Wild Korean Raccoon Dogs (<i>Nyctereutes procyonoides</i>): Description of 13 Cases.

Veterinary sciences·2026

Same author

Tissue-Resident ILC2s Across Organs: Heterogeneity, Niche Crosstalk, and Shared Regulatory Circuits.

Immune network·2026

Same journal

Fluorescence Resonance Energy Transfer in Calf Thymus DNA from a Long-Lifetime Metal-Ligand Complex to Nile Blue.

Journal of biochemistry and molecular biology·2021

Same journal

RETRACTION: Modulation of the specific interaction of cardiolipin with cytochrome c by zwitterionic phospholipids in binary mixed bilayers: A (2)H- and (31)P-NMR Study.

Journal of biochemistry and molecular biology·2009

Same journal

Ectopic expression of mitochondria endonuclease Pnu1p from Schizosaccharomyces pombe induces cell death of the yeast.

Journal of biochemistry and molecular biology·2007

Same journal

Cell selectivity of an antimicrobial peptide melittin diastereomer with D-amino acid in the leucine zipper sequence.

Journal of biochemistry and molecular biology·2007

Same journal

AtHAP3b plays a crucial role in the regulation of flowering time in Arabidopsis during osmotic stress.

Journal of biochemistry and molecular biology·2007

Same journal

Inhibitory properties of nerve-specific human glutamate dehydrogenase isozyme by chloroquine.

Journal of biochemistry and molecular biology·2007

See all related articles

Search research articles

Related Experiment Video

Updated: May 16, 2026

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology

Published on: May 15, 2012

Combinatorial solid phase peptide synthesis and bioassays.

Dong-Sik Shin¹, Do-Hyun Kim, Woo-Jae Chung

¹School of Chemical and Biological Engineering, Seoul National University, Korea.

Journal of Biochemistry and Molecular Biology

|October 6, 2005

Summary

This summary is machine-generated.

Solid phase peptide synthesis (SPPS) technologies enable combinatorial chemistry for creating peptide libraries. These libraries are crucial for high-throughput bioassays, including screening for drug discovery and understanding biological signaling pathways.

More Related Videos

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation

Published on: January 26, 2016

An Inexpensive Adaptation of a Commercial Microwave Reactor for Solid Phase Peptide Synthesis

An Inexpensive Adaptation of a Commercial Microwave Reactor for Solid Phase Peptide Synthesis

Published on: November 22, 2024

Related Experiment Videos

Last Updated: May 16, 2026

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology

Published on: May 15, 2012

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation

Published on: January 26, 2016

An Inexpensive Adaptation of a Commercial Microwave Reactor for Solid Phase Peptide Synthesis

An Inexpensive Adaptation of a Commercial Microwave Reactor for Solid Phase Peptide Synthesis

Published on: November 22, 2024

Area of Science:

Peptide Chemistry
Combinatorial Chemistry
Biotechnology

Background:

Solid phase peptide synthesis (SPPS), pioneered by Merrifield, revolutionized peptide chemistry and combinatorial approaches.
Peptide libraries are essential tools for modern biological and pharmaceutical research.
Advancements in synthesis technologies have expanded the scope and application of peptide libraries.

Purpose of the Study:

To review current solid phase peptide synthesis (SPPS) technologies relevant to combinatorial chemistry.
To highlight advancements in peptide library synthesis, including microarray techniques.
To discuss the applications of peptide libraries in high-throughput bioassays.

Main Methods:

Review of conventional SPPS methods: parallel synthesis, split-and-mix, and reagent mixture synthesis.
Exploration of peptide microarray synthesis on planar supports using surface chemistry and microelectronic fabrication.
Discussion of two microarray methodologies: pre-synthesized peptide immobilization and in situ synthesis (photolithography, SPOT method).

Main Results:

SPPS technologies have evolved significantly, supporting diverse peptide library synthesis strategies.
Peptide microarrays offer spatially addressable libraries for advanced applications.
Various SPPS methods facilitate the creation of large-scale peptide libraries.

Conclusions:

SPPS remains a cornerstone for combinatorial chemistry, enabling the generation of diverse peptide libraries.
Peptide microarrays represent a significant advancement, allowing for high-density, spatially defined peptide presentation.
Peptide libraries are indispensable for high-throughput screening in drug discovery and biological research.