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Related Experiment Videos

A "do-it-yourself" array biosensor.

Joel Golden1, Lisa Shriver-Lake, Kim Sapsford

  • 1Naval Research Laboratory, Code 6900, SW, Washington, DC 20375-5348, USA. jgolden@cbmse.nrl.navy.mil

Methods (San Diego, Calif.)
|October 6, 2005
PubMed
Summary

We created a fast array biosensor for detecting multiple targets in various samples. This innovative system offers sensitive detection comparable to ELISA, enabling rapid analysis in 15-30 minutes.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Optics

Background:

  • Simultaneous detection of multiple targets in multiple samples is crucial for diagnostics and security.
  • Existing methods like ELISA can be time-consuming and require complex sample preparation.
  • There is a need for rapid, sensitive, and versatile detection platforms.

Purpose of the Study:

  • To develop and characterize a novel array biosensor for high-throughput, simultaneous detection.
  • To demonstrate the biosensor's capability in detecting a wide range of targets.
  • To evaluate the performance of the biosensor in terms of speed and sensitivity.

Main Methods:

  • Utilized a planar waveguide (modified microscope slide) with patterned sensing regions.
  • Employed a 635 nm diode laser and a line generator for waveguide illumination.

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  • Measured fluorescence using a Peltier-cooled CCD camera to detect bound fluorophores.
  • Performed assays in multichannel flow chambers for multiplexed analysis.

    • Main Results:

    • Achieved simultaneous detection of multiple targets in multiple samples within 15-30 minutes.
    • Demonstrated detection limits rivaling those of the Enzyme-Linked Immunosorbent Assay (ELISA).
    • Successfully detected diverse targets including proteins, toxins, cells, viruses, and explosives.

    Conclusions:

    • The developed array biosensor provides a rapid and sensitive platform for multiplexed detection.
    • The system's versatility allows for application in various fields, from medical diagnostics to security.
    • This technology offers a significant advancement over conventional detection methods.