Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A carbon fiber microelectrode-based third-generation biosensor for superoxide anion.

Yang Tian1, Lanqun Mao, Takeyoshi Okajima

  • 1Department of Electronic Chemistry, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8502, Japan.

Biosensors & Bioelectronics
|October 6, 2005
PubMed
Summary

A novel implantable biosensor using carbon fiber microelectrodes (CFME) was developed for detecting superoxide anion (O2-). This advanced sensor shows high selectivity and sensitivity, making it suitable for in vivo measurements.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatiotemporally Precise Chemical Neuromodulation through MOF-Mediated Near-Infrared Control of Endogenous Signaling.

Journal of the American Chemical Society·2026
Same author

Bioinspired Nanofluidic Memristors Based on Polyelectrolyte Conformation for Synaptic Learning and in-Memory Logic Computing.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Octadecylamine-Modified CuO NPs Enabling Highly Selective <i>In Vivo</i> Ascorbic Acid Potentiometric Detection with Enhanced Sulfide Tolerance.

ACS sensors·2026
Same author

"Molecule-Selective Electrodes": Potentiometry Going Beyond Ions.

ACS sensors·2026
Same author

A Single-Vesicle Electroanalytical Approach Uncovers Deficits of Vesicular Acetylcholine Content in Alzheimer's Disease.

Journal of the American Chemical Society·2026
Same author

Confined-hydrogel fluidic memristor crossbar array for neuromorphic computing.

Nature communications·2026

Area of Science:

  • Electrochemistry
  • Biosensor Technology
  • Biomedical Engineering

Background:

  • Superoxide anion (O2-) is a crucial reactive oxygen species involved in various physiological and pathological processes.
  • Accurate and real-time detection of O2- is essential for understanding cellular functions and disease mechanisms.
  • Existing methods for O2- detection often lack the sensitivity, selectivity, or in vivo applicability required for comprehensive studies.

Purpose of the Study:

  • To develop and characterize a novel, implantable third-generation biosensor for the detection of superoxide anion (O2-).
  • To utilize carbon fiber microelectrodes (CFMEs) for enhanced electrochemical performance and in vivo compatibility.
  • To evaluate the analytical performance of the fabricated biosensor for O2- determination.

Main Methods:

Related Experiment Videos

  • Fabrication of CFME-based biosensors via electro-deposition of gold nanoparticles (AuNPs).
  • Modification of AuNPs with cysteine to promote electron transfer and immobilization of superoxide dismutase (SOD).
  • Electrochemical characterization and performance evaluation of the biosensor for O2- detection.

Main Results:

  • Successfully fabricated a CFME-based biosensor for O2- detection with direct electrochemistry facilitated by cysteine.
  • Demonstrated excellent analytical properties including optional operating potentials, high selectivity, high sensitivity, and good stability.
  • The biosensor exhibited inherent implantable capacity due to the CFME platform.

Conclusions:

  • The developed CFME-based biosensor represents a significant advancement for O2- detection.
  • Its striking analytical properties and implantable nature make it a promising tool for in vivo O2- monitoring.
  • This technology holds potential for applications in biological research and clinical diagnostics requiring real-time O2- analysis.