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[Evaluation of lymphocyte transformation test: relationship between nucleus and cell sizes of PHA-stimulated
Allergie Und Immunologie
|January 1, 1979
Abstract:
A methodological comparision of evaluation of lymphocyte transformation test is described. Three experts examined smear-slides of cells from five lymphocyte cultures and the corresponding controls in a blind study. In the morphological differentiation of 100 cells per slide in comparison to 1 000 cells per slide it was found a good intraindividually but a unsatisfactory interindividually correlation. 1 000 cells from stimulated and non-stimulated cultures shows the greatest difference for the parameter "total cell size" and the lowest difference for the "nucleus plasma relation" by the planimetrically imeasurement.