Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Expressible molecular colonies.

Timur R Samatov1, Helena V Chetverina, Alexander B Chetverin

  • 1Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia.

Nucleic Acids Research
|October 6, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Probing the legitimate initiation of RNA synthesis by Qβ replicase with oligonucleotide primers.

FEBS letters·2024
Same author

Slippage at the initiation of RNA synthesis by Qβ replicase results in a periodic polyG pattern.

FEBS letters·2022
Same author

Fra-2 overexpression upregulates pro-metastatic cell-adhesion molecules, promotes pulmonary metastasis, and reduces survival in a spontaneous xenograft model of human breast cancer.

Journal of cancer research and clinical oncology·2021
Same author

Alexander Spirin on Molecular Machines and Origin of Life.

Biochemistry. Biokhimiia·2021
Same author

Xenograft-derived mRNA/miR and protein interaction networks of systemic dissemination in human prostate cancer.

European journal of cancer (Oxford, England : 1990)·2020
Same author

Knockdown of L1CAM significantly reduces metastasis in a xenograft model of human melanoma: L1CAM is a potential target for anti-melanoma therapy.

PloS one·2018
Same journal

Correction to 'New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress'.

Nucleic acids research·2026
Same journal

VeloRM: disentangling pre- and post-splicing RNA modification dynamics at single-cell resolution.

Nucleic acids research·2026
Same journal

Accessibility of telomeric overhangs to stabilizing small-molecule ligands.

Nucleic acids research·2026
Same journal

Multivalent interactions mediate SNAIL transcription factor stimulation of the nucleosome deacetylase activity of the CoREST complex.

Nucleic acids research·2026
Same journal

Genome-wide mapping of DNA G-quadruplexes in Trypanosoma brucei chromatin reveals enrichment in coding regions and transcription start sites.

Nucleic acids research·2026
Same journal

Correction to 'The Gene Ontology knowledgebase in 2026'.

Nucleic acids research·2026
See all related articles

Researchers created artificial cell-like compartments in a gel for gene expression. This in vitro system allows for direct functional testing of cloned genes and their protein products within these nucleoprotein colonies.

Area of Science:

  • Molecular Biology
  • Synthetic Biology
  • Biochemistry

Background:

  • Polymerase chain reaction (PCR) in gels creates DNA colonies of genetic clones.
  • Existing methods often require separate steps for gene expression and analysis.

Purpose of the Study:

  • To demonstrate that transcription, translation, and protein folding can occur within the same gel environment.
  • To develop a cell-free system for in vitro gene expression and functional analysis.

Main Methods:

  • Performing polymerase chain reaction (PCR) within a gel matrix.
  • Integrating transcription, translation, and protein folding in the same gel.
  • Utilizing nucleoprotein colonies as compartmentalized reaction centers.

Main Results:

Related Experiment Videos

  • Successfully achieved simultaneous gene expression processes (transcription, translation, protein folding) in a gel.
  • Formed nucleoprotein colonies that compartmentalize synthesized RNAs and proteins with their DNA templates.
  • Demonstrated direct functional testing of cloned genes due to the absence of penetration barriers.

Conclusions:

  • Nucleoprotein colonies in gels can mimic cellular compartments for in vitro biological processes.
  • This system enables direct functional assessment of gene expression products.
  • The in vitro approach offers a versatile platform for gene and protein manipulation.