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Updated: Aug 15, 2026

Studying Cryptosporidium Infection in 3D Tissue-derived Human Organoid Culture Systems by Microinjection
Published on: September 14, 2019
Effect of bovine manure on Cryptosporidium parvum oocyst attachment to soil
Ewa Kuczynska1, Daniel R Shelton, Yakov Pachepsky
1Bldg. 173, Beltsville Agricultural Research Center East, 10300 Baltimore Ave., Beltsville, MD 20705, USA.
Abstract:
The objective of this work was to assess the effect of dilute bovine manure (1.0% and 0.1%) versus that of no manure on attachment and subsequent detachment of Cryptosporidium parvum oocysts to soil. Manure enhanced the attachment of oocysts to soil particles; the maximum attachment was observed with 0.1% manure. Oocyst attachment was partially reversible; maximum detachment was observed with dilute manure. These results indicate that oocyst attachment to soil is substantially affected by bovine manure in a complex manner and should have implications for how oocysts may be transported through or over soils.

