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Related Experiment Videos

Leukocyte-versus microparticle-mediated tissue factor transfer during arteriolar thrombus development.

Peter L Gross1, Barbara C Furie, Glenn Merrill-Skoloff

  • 1Center for Hemostasis and Thrombosis Research, Beth Israel Deaconess Medical Center and Harvard Medical School, Medicine, 330 Brookline Avenue, Boston, MA 02215, USA.

Journal of Leukocyte Biology
|October 6, 2005
PubMed
Summary

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Hematopoietic cell-derived tissue factor rapidly accumulates in developing thrombi via microparticles, not leukocytes. This finding clarifies the initial delivery mechanism of tissue factor in thrombus formation.

Area of Science:

  • Hematology
  • Vascular Biology
  • Cellular Biology

Background:

  • Circulating tissue factor (TF) is crucial for fibrin clot formation in developing thrombi.
  • The precise source and delivery mechanism of TF from hematopoietic cells to thrombi remain unclear.
  • Potential sources include TF-bearing microparticles and leukocytes expressing TF on their surface.

Purpose of the Study:

  • To investigate the kinetics of TF accumulation in developing thrombi.
  • To determine whether TF is delivered by microparticles or leukocytes.
  • To elucidate the temporal relationship between TF delivery and cellular interactions in thrombus formation.

Main Methods:

  • Intravital high-speed widefield and confocal microscopy in living mice.
  • Comparison of TF accumulation kinetics with leukocyte-thrombus and microparticle-thrombus interactions.

Related Experiment Videos

  • Assessment of P-selectin and P-selectin glycoprotein ligand-1 involvement in leukocyte adhesion.

    • Main Results:

    • TF rapidly accumulated in thrombi post-injury, peaking around 100 seconds.
    • Leukocyte-thrombus interactions were significantly delayed, observed after 2-3 minutes.
    • Microparticle accumulation in thrombi was rapid, peaking within 60 seconds.
    • TF accumulation kinetics correlated with microparticle kinetics, not leukocyte interactions.

    Conclusions:

    • Hematopoietic cell-derived TF is primarily delivered to developing thrombi via microparticles.
    • Microparticle-mediated TF delivery occurs during the initial phase of thrombus development.
    • Leukocyte-mediated TF contribution is temporally distinct from the early phase of thrombus formation.