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[Direct bone morphogenetic protein 2 gene therapy for repairing segmental radial defect in rabbits]
Jianjun Li1, Lunhao Bai, Hongbin Sun
1Department of Orthopaedics, the Second Hospital of China Medical University, Liaoning Shenyang, 110003, P. R. China. lijianjun71@yahoo.com.cn
Objective:
To study the effect of direct bone morphogenetic protein 2 (BMP-2) gene therapy mediated by adenovirus on repairing bone defect.
Methods:
The radial defect models were made on 60 rabbits, which were evenly divided into 4 groups randomly. The 4 groups were treated with different materials: group A, adenovirus carrying BMP-2 gene (Ad-BMP-2) plus bovine cancellous bone (BCB); group B, reconstructed BMP-2 plus BCB; group C, Ad-Lacz plus BCB; and group D, only BCB scaffolds. The X-ray, histological examination, biomechanics analysis, and immunohistochemical staining were made 4, 8, and 12 weeks after the operation.
Results:
Group A gained better effect in the volume of new bones, the anti-bending intensity of the healing bone, and the expression of BMP-2 than those of group B. The defect in group A was healed. No new bones were observed in group C and group D.
Conclusion:
Direct BMP-2 gene therapy is easy to perform and has very strong osteo-induction ability. It is a good method to repair segmental bone defects.
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