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High resolution of multiple forms of red blood cell enzymes using a Toyopearl DEAE 650 S
V Stocchi1, L Masat, B Biagiarelli
1Istituto di Chimica Biologica, Giorgio Fornaini, Università degli Studi, Urbino, Italy.
Preparative Biochemistry
|March 1, 1992
Abstract:
We have investigated a new anion exchange chromatographic support (Toyopearl DEAE 650 S) which simultaneously allows easily to remove hemoglobin from hemolysates and to obtain a very high resolution of enzymes present in multiple forms. The results obtained are better than those obtainable using an anion-exchange HPLC column. The data obtained at analytical level suggest a wider use of this new matrix also for preparative purposes without significant changes in the resolution.