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Crystallization and preliminary X-ray diffraction analysis of staphylococcal enterotoxin type C.

G A Bohach¹, Y I Chi, C V Stauffacher

¹Department of Bacteriology and Biochemistry, University of Idaho, Moscow 83843.

|April 1, 1992

Summary

Researchers crystallized Type C staphylococcal enterotoxin from Staphylococcus aureus. This advancement enables further structural studies of this important bacterial toxin.

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Area of Science:

Biochemistry
Structural Biology
Microbiology

Background:

Staphylococcus aureus produces staphylococcal enterotoxins, potent toxins implicated in food poisoning and other illnesses.
Type C staphylococcal enterotoxin is a significant virulence factor requiring detailed structural investigation.

Purpose of the Study:

To crystallize Type C staphylococcal enterotoxin for structural analysis.
To obtain high-quality crystals suitable for X-ray diffraction.

Main Methods:

Crystallization of the enterotoxin using ammonium sulfate, polyethylene glycol 400, and detergent.
Obtaining and characterizing triclinic and tetragonal crystal forms.

Main Results:

Two distinct crystal forms (triclinic and tetragonal) were successfully obtained.

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Crystals exhibited stability in X-ray beams and diffracted to high resolutions (2.2 and 2.6 A).

Detailed unit cell parameters and space group information were determined for both crystal forms.

Conclusions:

The successful crystallization of Type C staphylococcal enterotoxin provides a foundation for future structure-based drug design.
These crystal forms are suitable for determining the high-resolution structure of the toxin.