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Development of a nested qualitative real-time PCR assay to detect Aspergillus species DNA in clinical specimens

Catriona Halliday1, Qi Xuan Wu, Gregory James

  • 1NHMRC Clinical Center of Research Excellence in Infectious Diseases, Hematology, and Health Ethics, University of Sydney Western Clinical School, New South Wales 2145, Australia. catrionah@icpmr.wsahs.nsw.gov.au

Journal of Clinical Microbiology
|October 7, 2005
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...