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Related Experiment Videos

Developmental differences in megakaryocyte maturation are determined by the microenvironment.

William B Slayton1, David A Wainman, Xiao Miao Li

  • 1University of Florida Department of Pediatrics, Gainesville, Florida 32610, USA. slaytwb@peds.ufl.edu

Stem Cells (Dayton, Ohio)
|October 8, 2005
PubMed
Summary

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Neonatal stem cells produce smaller, less mature megakaryocytes, potentially explaining delayed platelet engraftment after umbilical cord blood transplants. Mouse studies show environmental factors also influence megakaryocyte development.

Area of Science:

  • Hematology
  • Stem Cell Biology
  • Transplantation Immunology

Background:

  • Delayed platelet engraftment post-umbilical cord blood transplant is often attributed to lower stem cell numbers.
  • Emerging evidence suggests neonatal stem cells may have an intrinsic limitation in producing mature, polyploid megakaryocytes.

Purpose of the Study:

  • To investigate whether intrinsic properties of neonatal stem cells or the microenvironment influence megakaryocyte maturation and ploidy.
  • To compare megakaryocyte development from neonatal versus adult hematopoietic stem and progenitor cells post-transplantation in mice.

Main Methods:

  • Hematopoietic stem and progenitor cells from transgenic mice (expressing green fluorescent protein) were isolated from adult bone marrow and newborn liver.
  • These cells were transplanted into myeloablated wild-type recipient mice.

Related Experiment Videos

  • Megakaryocyte size and ploidy were analyzed at various time points post-transplant.

    • Main Results:

    • Both neonatal and adult stem cells produced megakaryocytes larger than normal adult megakaryocytes early post-transplant.
    • Megakaryocytes derived from neonatal cells were significantly smaller and had lower ploidy than those from adult cells at 18 days post-transplant.
    • Differences in size and ploidy diminished by 1 month, with the largest megakaryocytes observed in the spleen.

    Conclusions:

    • Mouse studies indicate that both cell-intrinsic factors and the transplantation microenvironment contribute to maturational differences in neonatal versus adult megakaryocytes.
    • These findings suggest that differences in engraftment and response to thrombocytopenic stress by neonatal and adult megakaryocyte progenitors may underlie delayed platelet engraftment in cord blood transplantation.