Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

HER2 amplification and overexpression is not present in pediatric osteosarcoma: a tissue microarray study.

Gino R Somers1, Michael Ho, Maria Zielenska

  • 1Department of Pediatric Laboratory Medicine, Hospital for Sick Children, 555 University Avenue, Toronto, ON, M5G 1X8, Canada. gino.somers@sickkids.ca

Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society
|October 8, 2005
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of Genomic Mutations on the Transcriptional Pathways and Tumor Microenvironment Landscape of Localized Early Prostate Cancer.

The Prostate·2026
Same author

Risk factor profiles and haemodynamic progression in aortic stenosis: a retrospective population-based study.

Heart (British Cardiac Society)·2026
Same author

Effect of Anti-Siphon Devices on Postoperative Outcomes in Idiopathic Normal Pressure Hydrocephalus.

World neurosurgery·2026
Same author

Daneman Syndrome Revisited.

The Journal of pediatrics·2026
Same author

In reply to the Letter to Editor regarding "Head and neck cancer risk predictive models: a solution to streamline urgent suspected cancer referrals?"

The British journal of oral & maxillofacial surgery·2026
Same author

Usefulness of 3D joint space width on weight-bearing CT in comparison with 2D joint space width on radiographs for predicting 24-month worsening of knee osteoarthritis pain and function in the MOST study.

Osteoarthritis and cartilage·2025

HER2 gene amplification and protein overexpression were not detected in pediatric osteosarcoma samples. These findings suggest HER2-targeted therapies may not benefit this patient group.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • HER2 gene amplification and overexpression are common in breast cancer, indicating poor prognosis.
  • HER2 expression in osteosarcoma shows conflicting results, with varied prevalence and prognostic implications.

Purpose of the Study:

  • To investigate HER2 protein expression and gene amplification in pediatric osteosarcoma.
  • To evaluate the potential of HER2-targeted therapies in osteosarcoma treatment.

Main Methods:

  • Utilized tissue microarrays with 34 osteosarcoma samples (biopsies, resections, metastases).
  • Analyzed HER2 expression via immunohistochemistry and gene copy number using chromogenic in situ hybridization.

Main Results:

Related Experiment Videos

  • HER2 protein expression was observed focally and weakly in 12% of samples (2/18 patients).
  • No immunohistochemical overexpression (complete membrane positivity) or HER2 gene amplification was detected.
  • Tissue microarrays proved valuable for screening protein and gene expression.

    • Conclusions:

    • Pediatric osteosarcomas rarely exhibit HER2 overexpression or gene amplification.
    • HER2-targeted therapies are unlikely to be effective for pediatric osteosarcoma.
    • Tissue microarrays are effective tools for tumor screening at protein and gene levels.